A Phytophthora palmivora Extracellular Cystatin-Like Protease Inhibitor Targets Papain to Contribute to Virulence on Papaya.
Identifieur interne : 000873 ( Main/Exploration ); précédent : 000872; suivant : 000874A Phytophthora palmivora Extracellular Cystatin-Like Protease Inhibitor Targets Papain to Contribute to Virulence on Papaya.
Auteurs : Rebecca Gumtow [États-Unis] ; Dongliang Wu [États-Unis] ; Janice Uchida [États-Unis] ; Miaoying Tian [États-Unis]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2018.
Descripteurs français
- KwdFr :
- Carica (effets des médicaments et des substances chimiques), Carica (microbiologie), Espace extracellulaire (métabolisme), Fruit (microbiologie), Inhibiteurs de protéases (composition chimique), Inhibiteurs de protéases (pharmacologie), Maladies des plantes (microbiologie), Mutation (génétique), Papaïne (métabolisme), Phytophthora (effets des médicaments et des substances chimiques), Phytophthora (pathogénicité), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Virulence (effets des médicaments et des substances chimiques), Édition de gène (MeSH).
- MESH :
- composition chimique : Inhibiteurs de protéases.
- effets des médicaments et des substances chimiques : Carica, Phytophthora, Virulence.
- génétique : Mutation.
- microbiologie : Carica, Fruit, Maladies des plantes.
- métabolisme : Espace extracellulaire, Papaïne.
- pathogénicité : Phytophthora.
- pharmacologie : Inhibiteurs de protéases.
- Séquence d'acides aminés, Séquence nucléotidique, Édition de gène.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Carica (drug effects), Carica (microbiology), Extracellular Space (metabolism), Fruit (microbiology), Gene Editing (MeSH), Mutation (genetics), Papain (metabolism), Phytophthora (drug effects), Phytophthora (pathogenicity), Plant Diseases (microbiology), Protease Inhibitors (chemistry), Protease Inhibitors (pharmacology), Virulence (drug effects).
- MESH :
- chemical , chemistry : Protease Inhibitors.
- chemical , metabolism : Papain.
- drug effects : Carica, Phytophthora, Virulence.
- genetics : Mutation.
- metabolism : Extracellular Space.
- microbiology : Carica, Fruit, Plant Diseases.
- pathogenicity : Phytophthora.
- chemical , pharmacology : Protease Inhibitors.
- Amino Acid Sequence, Base Sequence, Gene Editing.
Abstract
Papaya fruits, stems, and leaves are rich in papain, a cysteine protease that has been shown to mediate plant defense against pathogens and insects. Yet the oomycete Phytophthora palmivora is a destructive pathogen that infects all parts of papaya plants, suggesting that it has evolved cysteine protease inhibitors to inhibit papain to enable successful infection. Out of five putative extracellular cystatin-like cysteine protease inhibitors (PpalEPICs) from P. palmivora transcriptomic sequence data, PpalEPIC8 appeared to be unique to P. palmivora and was highly induced during infection of papaya. Purified recombinant PpalEPIC8 strongly inhibited papain enzyme activity, suggesting that it is a functional cysteine protease inhibitor. Homozygous PpalEPIC8 mutants were generated using CRISPR/Cas9-mediated gene editing via Agrobacterium-mediated transformation (AMT). Increased papain sensitivity of in-vitro growth and reduced pathogenicity during infection of papaya fruits were observed for the mutants compared with the wild-type strain, suggesting that PpalEPIC8, indeed, plays a role in P. palmivora virulence by inhibiting papain. This study provided genetic evidence demonstrating that plant-pathogenic oomycetes secrete cystatins as important weapons to invade plants. It also established an effective gene-editing system for P. palmivora by the combined use of CRISPR/Cas9 and AMT, which is expected to be applicable to other oomycetes.
DOI: 10.1094/MPMI-06-17-0131-FI
PubMed: 29068239
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Tian">Miaoying Tian</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, 96822, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, 96822</wicri:regionArea><wicri:noRegion>96822</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Carica (drug effects)</term><term>Carica (microbiology)</term><term>Extracellular Space (metabolism)</term><term>Fruit (microbiology)</term><term>Gene Editing (MeSH)</term><term>Mutation (genetics)</term><term>Papain (metabolism)</term><term>Phytophthora (drug effects)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (microbiology)</term><term>Protease Inhibitors (chemistry)</term><term>Protease Inhibitors (pharmacology)</term><term>Virulence (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Carica (effets des médicaments et des substances chimiques)</term><term>Carica (microbiologie)</term><term>Espace extracellulaire (métabolisme)</term><term>Fruit (microbiologie)</term><term>Inhibiteurs de protéases (composition chimique)</term><term>Inhibiteurs de protéases (pharmacologie)</term><term>Maladies des plantes (microbiologie)</term><term>Mutation (génétique)</term><term>Papaïne (métabolisme)</term><term>Phytophthora (effets des médicaments et des substances chimiques)</term><term>Phytophthora (pathogénicité)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Virulence (effets des médicaments et des substances chimiques)</term><term>Édition de gène (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Protease Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Papain</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Inhibiteurs de protéases</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Carica</term><term>Phytophthora</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Carica</term><term>Phytophthora</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mutation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mutation</term></keywords><keywords 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Sequence</term><term>Base Sequence</term><term>Gene Editing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Édition de gène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Papaya fruits, stems, and leaves are rich in papain, a cysteine protease that has been shown to mediate plant defense against pathogens and insects. Yet the oomycete Phytophthora palmivora is a destructive pathogen that infects all parts of papaya plants, suggesting that it has evolved cysteine protease inhibitors to inhibit papain to enable successful infection. Out of five putative extracellular cystatin-like cysteine protease inhibitors (PpalEPICs) from P. palmivora transcriptomic sequence data, PpalEPIC8 appeared to be unique to P. palmivora and was highly induced during infection of papaya. Purified recombinant PpalEPIC8 strongly inhibited papain enzyme activity, suggesting that it is a functional cysteine protease inhibitor. Homozygous PpalEPIC8 mutants were generated using CRISPR/Cas9-mediated gene editing via Agrobacterium-mediated transformation (AMT). Increased papain sensitivity of in-vitro growth and reduced pathogenicity during infection of papaya fruits were observed for the mutants compared with the wild-type strain, suggesting that PpalEPIC8, indeed, plays a role in P. palmivora virulence by inhibiting papain. This study provided genetic evidence demonstrating that plant-pathogenic oomycetes secrete cystatins as important weapons to invade plants. It also established an effective gene-editing system for P. palmivora by the combined use of CRISPR/Cas9 and AMT, which is expected to be applicable to other oomycetes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29068239</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>07</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>31</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>03</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>A Phytophthora palmivora Extracellular Cystatin-Like Protease Inhibitor Targets Papain to Contribute to Virulence on Papaya.</ArticleTitle><Pagination><MedlinePgn>363-373</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-06-17-0131-FI</ELocationID><Abstract><AbstractText>Papaya fruits, stems, and leaves are rich in papain, a cysteine protease that has been shown to mediate plant defense against pathogens and insects. Yet the oomycete Phytophthora palmivora is a destructive pathogen that infects all parts of papaya plants, suggesting that it has evolved cysteine protease inhibitors to inhibit papain to enable successful infection. Out of five putative extracellular cystatin-like cysteine protease inhibitors (PpalEPICs) from P. palmivora transcriptomic sequence data, PpalEPIC8 appeared to be unique to P. palmivora and was highly induced during infection of papaya. Purified recombinant PpalEPIC8 strongly inhibited papain enzyme activity, suggesting that it is a functional cysteine protease inhibitor. Homozygous PpalEPIC8 mutants were generated using CRISPR/Cas9-mediated gene editing via Agrobacterium-mediated transformation (AMT). Increased papain sensitivity of in-vitro growth and reduced pathogenicity during infection of papaya fruits were observed for the mutants compared with the wild-type strain, suggesting that PpalEPIC8, indeed, plays a role in P. palmivora virulence by inhibiting papain. This study provided genetic evidence demonstrating that plant-pathogenic oomycetes secrete cystatins as important weapons to invade plants. It also established an effective gene-editing system for P. palmivora by the combined use of CRISPR/Cas9 and AMT, which is expected to be applicable to other oomycetes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gumtow</LastName><ForeName>Rebecca</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, 96822, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Dongliang</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, 96822, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Uchida</LastName><ForeName>Janice</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, 96822, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Miaoying</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, 96822, U.S.A.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1003536</GrantID><Agency>USDA</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>01</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011480">Protease Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.2</RegistryNumber><NameOfSubstance UI="D010206">Papain</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029441" MajorTopicYN="N">Carica</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005110" MajorTopicYN="N">Extracellular Space</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000072669" MajorTopicYN="N">Gene Editing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010206" MajorTopicYN="N">Papain</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011480" MajorTopicYN="N">Protease Inhibitors</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>10</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29068239</ArticleId><ArticleId IdType="doi">10.1094/MPMI-06-17-0131-FI</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Gumtow, Rebecca" sort="Gumtow, Rebecca" uniqKey="Gumtow R" first="Rebecca" last="Gumtow">Rebecca Gumtow</name></noRegion><name sortKey="Tian, Miaoying" sort="Tian, Miaoying" uniqKey="Tian M" first="Miaoying" last="Tian">Miaoying Tian</name><name sortKey="Uchida, Janice" sort="Uchida, Janice" uniqKey="Uchida J" first="Janice" last="Uchida">Janice Uchida</name><name sortKey="Wu, Dongliang" sort="Wu, Dongliang" uniqKey="Wu D" first="Dongliang" last="Wu">Dongliang Wu</name></country></tree></affiliations></record>Pour manipuler ce document 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